Holographic histopathology enables fast, precise diagnostics


Schematic of the imaging of pathological tissue 3D structure by integrating optical diffraction tomography and automated stitching. Credit: Hugonnet et al., doi 10.1117/1.AP.3.2.026004

Histology is the research study of biological tissues at a tiny level. Also called tiny anatomy, histology is commonly utilized to supply medical diagnosis of cancer and other illness. For example, tissue samples gotten throughout surgical treatment may assist to figure out whether more surgical action is required, and even more surgical treatment might be prevented if a medical diagnosis can be quickly gotten throughout an operation.


Traditional techniques in histopathology are typically restricted to thin specimens and need chemical processing of the tissue to supply adequately high contrast for imaging, which slows the procedure. A current advance in histopathology removes the requirement for chemical staining and enables high-resolution imaging of thick tissue areas. As reported in Advanced Photonics, a global research study group just recently showed a 3D label-free quantitative stage imaging strategy that utilizes optical diffraction tomography to acquire volumetric imaging info. Automated sewing streamlines the image acquisition and analysis.

Optical diffraction tomography

Optical diffraction tomography is a microscopy strategy for rebuilding the refractive index of a tissue sample from its scattered field images gotten with different lighting angles. It enables label-free high contrast visualization of transparent samples. The complicated spread field transferred through the sample is very first recovered utilizing off-axis holography, then the spread fields gotten with different angle of illuminations are mapped in the Fourier space making it possible for the restoration of the sample refractive index.

Holographic histopathology enables fast, precise diagnostics
Volumetric histopathology of unlabeled 100-μm-thick pancreas tissue sample from a client with intraductal papillary neoplasm of bile duct in the liver. For the function of contrast, nearby tissues were prepared in thin tissue slides with standard H&E staining approach. (the 5th row, 400x zoom). Credit: Hugonnet et al., doi 10.1117/1.AP.3.2.026004.

An acknowledged restriction of optical diffraction tomography is because of the complicated circulation of refractive indexes, which leads to considerable optical aberration in the imaging of thick tissue. To conquer this restriction, the group utilized digital refocusing and automated stitching, making it possible for volumetric imaging of 100-um-thick tissues over a lateral field of vision of 2 mm x 1.75 mm while preserving a high resolution of 170 nm x 170 nm x 1400 nm. They showed that synchronised visualization of subcellular and mesoscopic structures in various tissues is made it possible for by high resolution integrated with a broad field of vision.

Fast, precise histopathology

The scientists showed the capability of their unique approach by imaging a range of various cancer pathologies: pancreatic neuroendocrine growth, intraepithelial neoplasia, and intraductal papillary neoplasm of bile duct. They imaged millimeter-scale, unstained, 100-μm-thick tissues at a subcellular 3D resolution, which made it possible for the visualization of specific cells and multicellular tissue architectures, equivalent to images gotten with standard chemically processed tissues. According to YongKuen Park, scientist at the Korea Advanced Institute of Science and Technology and senior author on the research study, “The images obtained with the proposed method enabled clear visualization of different morphological features in the various tissues allowing for recognition and diagnosis of precursor lesions and pathologies.”

Park keeps in mind that more research study is required, however the outcomes recommend excellent possible for fast, precise histopathology throughout surgical treatment: “More research is needed on sample preparation, reconstruction speed, and mitigation of multiple scattering. We expect optical diffraction tomography to provide faster and more precise diagnostics in histopathology and intraoperative pathology consultations.”


Nonlinear wave blending facilitates subwavelength imaging


More info:
Herve Hugonnet et al, Multiscale label-free volumetric holographic histopathology of thick-tissue slides with subcellular resolution, Advanced Photonics (2021). DOI: 10.1117/1.AP.3.2.026004

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Holographic histopathology enables fast, precise diagnostics (2021, April 30)
recovered 30 April 2021
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