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  1. [This](http://physiology.med.uvm.edu/warshaw/TechspgInVitro.html) is a good description of what’s going on. It also has this little sketch at the top, that shows the set up. If you add actin and myosin proteins and supply energy by ATP, myosin perform little ‘steps’ (actually called strokes) that transport actin. That’s what’s happening in muscles. Here, however, many myosins were fixed on a glass surface with their ‘head’ sticking upwards. Actin filaments will then be transported over the surface. Since the myosin filaments are randomly orientated, the movement is random too.

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